E. coli 0157:H7 Ecoli research

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What is PulseNet?

In 1993, a large outbreak of foodborne illness caused by E. coli O157:H7 occurred in the western United States. In this outbreak, scientists at the CDC performed DNA "fingerprinting" by PFGE and determined that the strain of E. coli O157:H7 found in patients had the same PFGE pattern as the strain isolated from hamburger patties served at a large chain of regional fast food restaurants. Prompt recognition of this outbreak and its cause may have prevented more than the estimated 800 illnesses. As a result, the CDC developed standardized PFGE methods and, in collaboration with the Association of Public Health Laboratories, created PulseNet so that scientists at public health laboratories throughout the country could rapidly compare the PFGE patterns of bacteria isolated from ill persons and determine whether they are similar.

PulseNet is an early warning system for outbreaks of foodborne disease. It is a national network of public health laboratories that performs DNA “fingerprinting” on bacteria that may be foodborne. The network identifies and labels each “fingerprint” pattern and permits rapid comparison of these patterns through an electronic database at the CDC to identify related strains.

At present, PulseNet tracks four foodborne disease-causing bacteria: E. coli O157:H7, nontyphoidal Salmonella, Shigella and Listeria monocytogenes.

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